Temperature-dependent germination and endo-beta -mannanase activity in sesame seeds
نویسندگان
چکیده
منابع مشابه
A germination-specific endo-beta-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds.
Endo-beta-mannanase (EC 3.2.1.78) is involved in hydrolysis of the mannan-rich cell walls of the tomato (Lycopersicon esculentum Mill.) endosperm during germination and post-germinative seedling growth. Different electrophoretic isoforms of endo-beta-mannanase are expressed sequentially in different parts of the endosperm, initially in the micropylar endosperm cap covering the radicle tip and s...
متن کاملEndo-β-mannanase Activity in Tomato and Other Ripening Fruits
High amounts of endo-β-mannanase (EC 3.2.1.78) activity were extracted from tomato (Lycopersicon esculentum Mill.) fruits when a high-salt-containing buffer was used. Two pI forms of the fruit enzyme were identified, one being much more basic than the many seed isoforms. The number of isoforms increased if a protease inhibitor was not used during extraction. The enzyme was found in the ripe fru...
متن کاملModular Hyperthermostable Bacterial Endo-β-1,4-Mannanase: Molecular Shape, Flexibility and Temperature-Dependent Conformational Changes
Endo-β-1,4-mannanase from Thermotoga petrophila (TpMan) is a hyperthermostable enzyme that catalyzes the hydrolysis of β-1,4-mannoside linkages in various mannan-containing polysaccharides. A recent study reported that TpMan is composed of a GH5 catalytic domain joined by a linker to a carbohydrate-binding domain. However, at this moment, there is no three-dimensional structure determined for T...
متن کاملThe emergence of embryos from hard seeds is related to the structure of the cell walls of the micropylar endosperm, and not to endo-beta-mannanase activity.
BACKGROUND AND AIMS Seeds of carob, Chinese senna, date and fenugreek are hard due to thickened endosperm cell walls containing mannan polymers. How the radicle is able penetrate these thickened walls to complete seed germination is not clearly understood. The objective of this study was to determine if radicle emergence is related to the production of endo-beta-mannanase to weaken the mannan-r...
متن کاملEndo-β-mannanase from the endosperm of seeds of Sesbania virgata (Cav.) Pers. (Leguminosae): purification, characterisation and its dual role in germination and early seedling growth
Abbreviations: ABA, abscisic acid; BSA, bovine serum albumin; CAP, micropylar area around the radicle emergence point or endosperm cap; DAB, diaminobenzidine; DEAE, diethylaminoethyl; IgG, immunoglobulin G; LAT, lateral coat plus endosperm; LBG, locust bean gum; ηsp, specific viscosity; PBS, phosphate buffer saline; pI, isoelectric point; RAD, radicle tip; SDS-PAGE, sodium dodecyl sulfate polya...
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ژورنال
عنوان ژورنال: Revista Brasileira de Fisiologia Vegetal
سال: 2001
ISSN: 0103-3131
DOI: 10.1590/s0103-31312001000200003